[Extraction and content determination of polysaccharides in Viscum coloratum].
Copyright policy )[Extraction and content determination of polysaccharides in Viscum coloratum].
To establish a method for extraction and content determination of polysaccharide in Viscum coloratum.Polysaccharide was extracted by hot water, separated by ultrafiltration and ion-exchange chromatography. The content determination was performed at wavelength 490 nm with phenol-sulfuric acid as a chtomo-genic agent.The content of polyaccharide in V. coloratum, CVPS-III, and CVPS-III-C were respectively 4.93% (RSD 1.04%, n = 3), 43.28% (RSD 1.39%, n =3), 69.55% (RSD 1.62%, n = 3), and the average recovery was 96.07% (RSD 2.54%, n = 5).The method was simple, rapid, and accurate.
- Jiangsu University of Science and Technology China (People's Republic of)
Viscum, Plants, Medicinal, Plant Stems, Polysaccharides, Reproducibility of Results, Chromatography, Ion Exchange
Viscum, Plants, Medicinal, Plant Stems, Polysaccharides, Reproducibility of Results, Chromatography, Ion Exchange
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