To evaluate the quality of Coptis chinensis planted with four kinds of cultivating system.The samples, which were planted below the artificial shading, the forest of Chinese ceder wood, Mmulbery wood, Magnolia officinal wood were chosen respectivley, UV and HPLC were used to determine of total alkaloids and berberine of samples.There was no significant difference among four kinds of cultivating system on the contents of total alkaloids and berberine. The contents of total alkaloids and berberine were obviously higher than in the criterion of Chinese Pharmacopoeia.The roots of C. chinensis planted in the four kinds of planting system can be used as medicinal material of high quality.