Maize (Zea mays L.).
Copyright policy )Maize (Zea mays L.).
Agrobacterium tumefaciens-mediated transformation is an effective method for introducing genes into maize. In this chapter, we describe a detailed protocol for genetic transformation of the maize genotype Hi II. Our starting plant material is immature embryos cocultivated with an Agrobacterium strain carrying a standard binary vector. In addition to step-by-step laboratory transformation procedures, we include extensive details in growing donor plants and caring for transgenic plants in the greenhouse.
- Iowa State University United States
Transformation, Genetic, Agrobacterium tumefaciens, Genetic Vectors, Gene Transfer Techniques, Plants, Genetically Modified, Zea mays, Coculture Techniques
Transformation, Genetic, Agrobacterium tumefaciens, Genetic Vectors, Gene Transfer Techniques, Plants, Genetically Modified, Zea mays, Coculture Techniques
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