6 duplicate floor pens with 20-day-old commercial broiler chicks each, were fed practical-type broiler diet with maize-palm kernel meal, supplemented with graded levels of biotin so that the rations had 40, 80, 120, 160, 200, and 240 micrograms of the vitamin per kg of feed. This treatment was maintained for 6 weeks. Estimation of the body weight, feed intake, blood glucose concentration, and lipid contents of liver, kidney and blood showed that dietary biotin of 120 micrograms/kg feed is a minimum requirement. 160 micrograms/kg feed was required for the prevention of dermatitis, mortality due to fatty liver and kidney syndrome (FLKS) and leg deformities.