Total lipid composition of liver oil of two marine fish Carcharhinus melanopterus (Black Shark) and Lutjanus johnii (Hira) was determined. The fatty acid contents of total liver lipids were identified through gas chromatography-mass spectrometry. It was found that fatty acid methyl eaten mainly consist of myristic, pentadecylic, palmitic, stearic acids as saturated, and palmitoleic, oleic, elaidic, eicosapentaenoic, docosahexaenoic, and clupanodonic acids as unsaturated components. Appreciable quantities of polyunsaturated fatty acids (PUPA) and omega-3 fatty acids were detected.