The paper aims to investigate the use of colour and its perception within the cabin of a regional aircraft used for short distances and it is divided into two parts. The first part presents an analysis of the literature and the state of the art on the use of colour both within the aeronautical sector and in comparable sectors, such as that of interior architecture, where over the last few decades researchers formulated different theories of approach. The case studies examined show that, today, the choice of using colour is mainly based on marketing factors linked to the corporate branding of airlines, with a lack of sensitivity to human factors. The literature on colour, on the other hand, suggests that the different tones of colour have a strong impact from a physiological and psychological point of view, factors that must be taken into consideration to improve the experience of wellbeing on board, defining a framework of requirements. The chromatic hypotheses were, then, examined and compared through a preliminary perceptual test conducted on a sample of 20 subjects aimed at evaluating and defining guidelines for approaching the colour project. The objective of the guidelines is the construction of chromatic design scenarios defining a comfortable environment from the visual point of view and capable not only of generating a pleasant, comfortable travel experience but also a feeling of security and limiting the typical unwanted perceptions related to flight. The paper is part of the dissemination of the H2020 CASTLE project (CAbin Systems design Toward passenger welLbEing).