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image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
Pamukkale GCRIS Database
Article . 2002
Data sources: Pamukkale GCRIS Database
image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
Pamukkale Üniversitesi Açık Erişim Arşivi
Article . 2002
Data sources: Pamukkale Üniversitesi Açık Erişim Arşivi
image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
Bursa Uludag University - AVESIS
Conference object . 2002
Data sources: Bursa Uludag University - AVESIS
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Bursa Uludag University - AVESIS
Article . 2002
Data sources: Bursa Uludag University - AVESIS
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Claim

Detection of infectious bronchitis virus in tracheal organ cultures using the immunoperoxidase technique

descriptionPublicationkeyboard_double_arrow_right Article , Conference object 01 Jan 2002 Turkey Turkish
Authors: Şen, A.; Özyigit, M.O.; Sönmez, G.; Caner, Vildan.;

handle: 11499/5339

Detection of infectious bronchitis virus in tracheal organ cultures using the immunoperoxidase technique

Abstract

The applicability of the immunoperoxidase (IP) technique in unfixed-fresh tracheal organ cultures (TOCs) was investigated for rapid detection of infectious bronchitis virus (IBV). For this purpose, TOCs prepared from one-day-old SPF chicks were inoculated with IBV M41 strain in serial dilutions. IBV antigen detected by IP technique in TOCs 18 h postinoculation. IP staining was observed before siliostasis in all dilutions. The IP technique was also applied to detect IBV antigen in formalin-fixed, paraffin-embedded sections of TOCs inoculated with IBV M41 strain. Viral antigen was also detected in these sections. Histopathological examinations of IBV M41 infected tracheal rings showed loss of cilia, flattening of lamina epithelialis, and rounding and desquamation of epithelial cells.

Country
Turkey
Related Organizations
Keywords

virus strain, chicken, Infectious bronchitis virus, Immunoperoxidase, peroxidase, trachea, Gallus gallus, 630, paraffin, animal tissue, organ culture, Tracheal organ cultures, newborn, 616, virus infection, inoculation, Animalia, controlled study, virus detection, nonhuman, article, antigen detection, virus antigen, tracheitis, Avian infectious bronchitis virus, histopathology, formaldehyde, animal disease, Aves

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    popularity
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    influence
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
Green