Educational buildings present complex layouts due to their programs, functional schemes, strict demarcation or uncertainty of circulation routes, and social spaces, especially if the building is a transformed one with various university-level disciplines packed together. In buildings housing disciplines that function differently, the use of spaces reveals some discordance in the layout. Moreover, the social behaviour of students also diversifies accordingly. As peer-to-peer learning is a crucial part of contemporary education, informal spaces play important roles to create this social bond through interactions, encounters, co-presence, and such in the gathering areas. The aim of this study is to explore whether the type of education, department’s architectural layout, and study programs have any effect on the students’ spatial preference in a university building. The case study area located on a university campus is part of a building that was originally a textile factory and showroom. The building houses the faculties of architecture and design, engineering, and English preparatory schools. Labyrinth-like configuration of the current use with many functions and different behaviour modes taking place at the same time displays emergent social interactions in the non-allocated spaces. To reveal the social gathering habits and preferences of the students, a structured questionnaire has been implemented. The results of the questionnaire showed the most frequently used areas and their purposes. Integration analysis of the floor plans, determined, identified and correlated with the perceptions and social gathering preferences of students showing that the habits of spatial uses differ according to the curriculum of the departments.