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Acidose ruminal subaguda em ovinos: flutuações diárias dos valores de pH ruminal, fecal e urinário e dinâmica do fluxo da artéria tibial cranial

Authors: Bueno, Gabriela Marchiori;

Acidose ruminal subaguda em ovinos: flutuações diárias dos valores de pH ruminal, fecal e urinário e dinâmica do fluxo da artéria tibial cranial

Abstract

RESUMO – A acidose ruminal subaguda (ARS) é caracterizada como doença metabólica de ruminantes alimentados com dieta de alta concentração de carboidratos e escassez de fibras, associada à diminuição do pH ruminal. As consequências da ARS incluem alteração do consumo alimentar, queda na produção, alteração na microbiota e digestão ruminal, diarreia, laminite, abscessos hepáticos e sepse. O presente estudo avaliou as flutuações diárias dos valores do pH ruminal, fecal e urinário, alterações no líquido ruminal e analisou a dinâmica do fluxo da artéria tibial cranial, por ultrassonografia Doppler. Foram utilizadas oito ovelhas adultas, hígidas, distribuídas em grupo tratado (GT, cinco animais, que receberam alta porcentagem de concentrado na dieta) e grupo controle (GC, três animais, alimentados com feno). Para a indução da ARS, as ovelhas do GT receberam dieta na qual foram incluídos ao volumoso, diariamente, 10% de alimento concentrado farelado até completar 80%, porcentagem mantida até finalizar os 30 dias experimentais. Diariamente foram mensurados o pH ruminal, fecal e urinário; no líquido ruminal foram avaliados os aspectos físicos (viscosidade, cor e odor), o tempo de sedimentação, prova de redução do azul de metileno e níveis de histamina. Posteriormente, realizou-se o exame ultrassonográfico Doppler nas artérias tibial cranial do membro pélvico esquerdo e direito. Os parâmetros Doppler analisados foram velocidade máxima sistólica, velocidade diastólica final, índice de pulsatilidade e resistividade. As ovelhas com ARS induzida (GT) apresentaram: diminuição do pH ruminal e aumento do pH fecal e urinário; o fluido ruminal apresentou coloração amarela, diminuição do tempo de redução do azul de metileno, aumento do tempo de sedimentação e diminuição dos níveis de histamina; as ovelhas não apresentaram sinais clínicos evidentes da laminite e nos parâmetros Doppler avaliados observou-se aumento da velocidade diastólica final e diminuição dos índices de pulsatilidade e resistividade na artéria tibial cranial de ambos os membros pélvicos das ovelhas. Concluiu-se que o incremento de carboidrato na dieta causou alterações no pH ruminal, fecal e urinário; no líquido ruminal e vasodilatação na da artéria tibial cranial.

Subacute ruminal acidosis (ARS) is characterized as a metabolic disease of ruminants fed a diet of high carbohydrate concentration and fiber shortage associated with decreased of ruminal pH. The consequences of ARS include altered food intake, decreased production, altered microbiota and ruminal digestion, diarrhea, laminitis, hepatic abscesses, and sepsis. The present study evaluated the daily fluctuations of ruminal, fecal and urinary pH values, alterations in ruminal fluid and analyzed the flow dynamics of the cranial tibial artery by Doppler ultrasonography. Eight healthy adult sheeps were distributed in treated group (TG, five animals, which received high percentage of concentrate in the diet) and control group (CG, three animals fed hay). For the ARS induction, sheeps from TG received a diet in which 10% of the concentrate feed was added to the bulk, until 80%, until the end of the 30 experimental days. Ruminal, fecal and urinary pH values were measured; in ruminal fluid the physical aspects (viscosity, color and odor), sedimentation time, methylene blue reduction test and histamine levels were evaluated. Subsequently, Doppler ultrasonographic examination was performed on the cranial tibial artery of left and right pelvic limbs. The Doppler parameters analyzed were maximum systolic velocity, end-diastolic velocity, pulsatility index and resistivity index. Sheeps with induced ARS (TG) presented: decreased of ruminal pH and increased of fecal and urinary pH; ruminal fluid presented yellow staining, decreased of time of methylene blue reduction test, increased of sedimentation time and decreased of histamine levels; the sheeps did not present evident clinical signs of laminitis and Doppler parameters evaluated showed an increase in final diastolic velocity and a decrease in pulsatility and resistivity indexes in the cranial tibial artery of both pelvic limbs. It was concluded that the increase of carbohydrate in diet caused changes in ruminal, fecal and urinary pH; in ruminal fluid and vasodilation in the cranial tibial artery.

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Pós-graduação em Medicina Veterinária - FCAV

Processo FAPESP: 2016/16631-8

Country
Brazil
Keywords

ovelhas, pH ruminal fluid, Laminitis, Laminite, Pulsatility index, pH fluido ruminal, Resistivity index, Índice de resistividade, Ewes, Índice de pulsatilidade, ARS, SARA

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
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