The palaeontological site of San Teodoro (Acquedolci, Messina, Italy) is one of the most important points for the study of palaeoanthropology and palaeoecology of the Final Pleistocene in the Mediterranean area. The cave in the final Epigravettian was used as a burial site and until now were found the human remains of 7 inhumations. New research activities conducted by LabHomo (University of Palermo) on human remains have focused on the digital acquisition of the specimens through the use of CT scans and processing through specific software following the principles of the virtual anthropology. In this contribution, the preliminary data obtained by analyzing the endocasts of the ST1 and ST2 individuals are presented. Were applied a combination of qualitative methodologies, for a general description of brain morphology, the pattern of venous system and the gyrification pattern, and quantitative methodologies (i.e., geometric morphometry) to investigate the cerebral asymmetries. The comparison collection consists of sixty Anatomical Modern Humans (taking in consideration age and sex variability). This preliminary study has shown that the brain morphology of the two individuals ST1 and ST2 site falls within the intraspecific variability of AMH, except for the right-frontal left-occipito petalia asymmetry, which is opposed to the modern human’s variability.