Entrepreneurship in the Healthcare sector has received increased attention over the last two decades, both in terms of scholarly research and number of innovative enterprises. Entrepreneurial activities and innovations have emerged from and will continue to be driven by several actors along the healthcare value chain but especially from non-traditional healthcare players. In this new volume, we present the reader with several critical issues in healthcare entrepreneurship and innovation, covering a comprehensive set of research topics. We bring together the latest academic research and management practice, with contributions by authors from entrepreneurship, medical sciences, and management, who provide in depth and practical insights into designing and managing entrepreneurship in healthcare. Upon providing a systematic review of the research field, we discuss several important macro-, meso-, and micro-level issues in healthcare entrepreneurship, such as opportunity identification, the entrepreneurial ecosystem including accelerators, the benefits of open innovation for the sector, and social entrepreneurship in healthcare.