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CONICET Digital
Article . 2021
License: CC BY SA
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The addition of Andean lupin (Lupinus mutabilis) protein concentrate enhances the nutritive value and the antioxidant activity of yoghurt

Authors: Curti, Carolina Antonela; De Carvalho Fino, Luciana; Olivares la Madrid, Ana Paula; Badan Ribeiro, Ana Paula; da Cunha, Diogo Thimoteo; Vinderola, Celso Gabriel; Costa Antunes, Adriane Elisabete; +1 Authors

The addition of Andean lupin (Lupinus mutabilis) protein concentrate enhances the nutritive value and the antioxidant activity of yoghurt

Abstract

Introducción: Las semillas de altramuces andinos son fuentes poco explotadas de nutrientes y compuestos bioactivos. Materiales y Métodos: Las semillas de altramuz se desamargaron para eliminar alcaloides tóxicos, se desgrasaron parcialmente con etanol al 70%, se secaron a 60 ° C y se molieron. La harina desgrasada se suspendió en agua ajustada a pH 7 con NaOH 1 N y el extracto de proteína se filtró y se secó por pulverización. Se obtuvieron yogures enriquecidos con la adición de concentrado de proteína de altramuz al 0,5, 1 y 1,5%. Se determinó la composición química y de ácidos grasos, el contenido de polifenoles y flavonoides, así como la actividad antioxidante. Se evaluó la calidad microbiana y las propiedades fisicoquímicas (valores de pH, contenido de ácido láctico y porcentaje de sinéresis) en yogures durante 28 días de almacenamiento. Resultados: El concentrado de altramuz andino presentó altos contenidos de proteínas y carbohidratos (69% y 20%), ácidos grasos insaturados (77%) principalmente ácidos oleico, linoleico y α-linolénico, así como bajos índices aterogénicos y trombogénicos (0,07 y 0,44). La adición de concentrado de proteína de altramuz al 1,5% en los yogures aumentó el contenido de proteína. Los yogures enriquecidos mostraron mayor contenido de ácidos grasos insaturados, polifenoles totales y flavonoides, así como índices aterogénicos y trombogénicos más bajos en comparación con el control. El concentrado de altramuz aumentó la actividad captadora de radicales ABTS, el poder reductor férrico y la actividad antioxidante total en yogures. Los yogures enriquecidos y control mostraron recuentos de bacterias de ácido láctico similares. Los recuentos de coliformes totales y termotolerantes se mantuvieron por debajo de tres NMP/mL, levaduras y mohos por debajo de 10 UFC/mL. Los valores de pH en los productos enriquecidos fueron mayores en los días 1 y 7 de almacenamiento (4,82 y 4,95) en comparación con la muestra control (4,72 y 4,85). El contenido de ácido láctico aumentó en todas las formulaciones al día 21. Los yogures enriquecidos mostraron menor sinéresis que el control. Conclusiones: Los yogures enriquecidos con concentrado de proteína de altramuz podrían representar alternativas nutritivas y funcionales para prevenir enfermedades crónicas al consumirse en una dieta saludable.

Introduction: Andean lupin seeds are under-exploited sources of nutrients and bioactive compounds. Materials and methods: Seeds were debittered to remove toxic alkaloids, partially defatted with 70% ethanol, dried at 60°C and milled. The defatted flour was suspended in water adjusted at pH 7 with IN NaOH. The protein extract was filtered and spray-dried. Enriched yoghurts were obtained with the addition of lupin protein concentrate at 0.5,1 and 1.5%. The chemical and fatty acid composition, the contents of polyphenols and flavonoids as well as the antioxidant activity were determined. The microbial quality and physicochemical properties (pH values, lactic acid content and percentage of syneresis) were evaluated in yoghurts during 28 days of storage. Results: Andean lupin concentrate showed high protein and carbohydrate contents (69% and 20%), unsaturated fatty acids (77%) mainly oleic, linoleic and a-linolenic acids as well as low atherogenic and thrombogenic indexes (0.07 and 0.44). The addition of the lupin protein concentrate at 1.5% into yoghurts increased the protein content. Enriched yoghurts showed higher unsaturated fatty acids, total polyphenol and flavonoid contents as well as lower atherogenic and thrombogenic indexes compared to the control. The lupin protein concentrate increased the ABTS radical scavenging activity, the ferric reducing power and the total antioxidant activity in yoghurts. Enriched and control yoghurts showed similar lactic acid bacterial counts. The counts for total and thermotolerant coliforms were under three MPN/mL and yeast and molds under 10 CFU/ mL. The pH values in enriched products were higher at days 1 and 7 of storage (A.82 and 4.95) compared to the control sample (4.72 and 4.85). The lactic acid content increased in all formulations at day 21. Enriched yoghurts showed lower syneresis than the control. Conclusions: Yoghurts enriched with lupin protein concentrate could represent nutritive and functional alternatives to prevent chronic diseases when consuming in a healthy diet.

Fil: Ramón, Adriana Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina. Universidad Nacional de Salta. Facultad de Ciencias de la Salud; Argentina

Fil: Olivares la Madrid, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina

Fil: Curti, Carolina Antonela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina

Fil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina

Fil: Costa Antunes, Adriane Elisabete. Universidade Estadual de Campinas; Brasil

Fil: De Carvalho Fino, Luciana. Universidade Estadual de Campinas; Brasil

Fil: Badan Ribeiro, Ana Paula. Universidade Estadual de Campinas; Brasil

Fil: da Cunha, Diogo Thimoteo. Universidade Estadual de Campinas; Brasil

Country
Argentina
Keywords

LUPINUS, RC620-627, PROTEINS, Nutrition. Foods and food supply, FLAVONOIDS, lupino; proteínas; ácidos grasos insaturados; polifenoles; flavonoides; índice aterogénico; enfermedad cardiovascular, POLYPHENOLS, https://purl.org/becyt/ford/2.11, TX341-641, UNSATURATED FATTY ACIDS, https://purl.org/becyt/ford/2, Nutritional diseases. Deficiency diseases

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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