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Article . 2016
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Pardeamiento enzimático: caracterización fenotípica, bioquímica y molecular en variedades de papa nativas de la Argentina

Authors: Suarez, Patricia Alejandra; Andreu, Adriana Balbina; Colman, Silvana Lorena; Clausen, Andrea Martina; Feingold, Sergio;

Pardeamiento enzimático: caracterización fenotípica, bioquímica y molecular en variedades de papa nativas de la Argentina

Abstract

El pardeamiento enzimático (PE) esta relacionado con la actividad de la enzima polifenol oxidasa (PPO) que cataliza la oxidación a diferentes compuestos fenólicos, con la consecuente transformación a pigmentos oscuros no deseables para la calidad industrial. Este trabajo contribuye a la caracterización del PE en papas nativas (Solanum tuberosum ssp. andigenum) a través de la determinación de la variabilidad alélica de los genes de PPO, la actividad enzimática relacionada y la resultante calidad industrial por medio de parámetros cualitativos. En los 20 genotipos estudiados se encontraron diferencias significativas en la actividad de PPO y el índice oxidativo (IO), lo que permitió distinguir individuos con mejor comportamiento frente al PE. Asimismo, se observó variabilidad genética en las regiones amplificadas de los genes de PPO identificándose 9 posibles variantes alélicas. Hasta el momento no se pudo establecer asociación entre bandas y la actividad de PPO o IO, posiblemente debido a factores ambientales que pudieron haber incidido durante el desarrollo del cultivo y/o al número reducido de genotipos estudiados a la fecha.

Enzymatic browning (EB) is related to polyphenol oxidase (PPO) activity, which catalyzes the oxidation of various phenolic compounds that renders dark pigments which are undesirable for industrial quality of potato. This study contributes to the characterization of EB in native potatoes (Solanum tuberosum ssp. andigenum) through the analysis of allelic variability in PPO genes, the determination of the enzymatic activity and the resulting darkening of tubers through qualitative parameters. Variability was found among 20 selected genotypes in activity of PPO and oxidative index (OI), which allowed distinguishing individuals with better EB performance. Also, genetic variability was observed in the amplified regions of PPO genes, which shown 9 possible allelic variants. However, no clear association was found between putative alleles and PPO activity or OI .This lack of association can be related to the reduced number of genotypes assayed up to date and/or environmental factors that could have influenced tuber production.

Fil: Colman, Silvana Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina

Fil: Suarez, Patricia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina

Fil: Andreu, Adriana Balbina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina

Fil: Feingold, Sergio. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Área de Investigación en Agronomía; Argentina

Fil: Clausen, Andrea Martina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Laboratorio de Genetica; Argentina

Country
Argentina
Keywords

índice oxidativo, https://purl.org/becyt/ford/4.4, pardeamiento enzimático, polifenol oxidasa, papas nativas, https://purl.org/becyt/ford/4

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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