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Article . 2015
License: CC BY NC SA
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Degradación y utilización de la hemicelulosa contenida en especies forrajeras por Pseudobutyrivibrio ruminis y Pseudobutyrivibrio xylanivorans

Authors: Grilli, Diego; Egea, Vanina; Paez Lama, Sebastián; Carcaño, Diego; Allegretti, Liliana; Sosa Escudero, Miguel; Arenas, Graciela Nora;

Degradación y utilización de la hemicelulosa contenida en especies forrajeras por Pseudobutyrivibrio ruminis y Pseudobutyrivibrio xylanivorans

Abstract

Este estudio fue desarrollado para determinar la degradación y utilización de la hemicelulosa contenida en diferentes forrajes por dos cepas bacterianas (P. xylanivorans 2 y P. ruminis 153), aisladas del rumen de caprinos Criollos. El cultivo de las cepas puras fue desarrollado hasta evidenciar la fermentación completa del heno de alfalfa y de diversas forrajeras nativas. La degradación fue definida como la solubilización de las pentosas insolubles en etanol al 80%, mientras que la utilización se definió como la pérdida de las pentosas totales. Los componentes nutricionales medidos en los forrajes evaluados fueron: cutina, fibra neutro detergente, fibra ácido detergente, lignina ácido detergente, hemicelulosa, celulosa, proteína cruda y almidón. A. lampa evidenció el máximo valor de degradación y utilización de la hemicelulosa y M. ephedroides y G. decorticans fueron las forrajeras nativas con el menor valor de degradación, en comparación con el heno de alfalfa. El coeficiente de correlación entre el valor de lignina ácido detergente y el porcentaje de degradación y utilización de la hemicelulosa, sugiere que la lignina afecta la actividad hemicelulolítica de P. xylanivorans. Estos resultados proporcionan un marco para intentar modificar la actividad hemicelulolítica del ambiente ruminal, mediante la incorporación de P. xylanivorans como cepa probiótica.

This study was performed to determine the degradation and utilization of hemicellulose from intact forages by two strains (P. xylanivorans 2 and P. ruminis 153) isolated from rumen of Creole goats. Fermentations by pure culture were run to completion by using alfalfa hay and native forages selected by goats grazing. Degradation was defined as the solubilization of 80% ethanol-insoluble pentose, whereas utilization was defined as a loss in total pentose. The nutritional components measured from intact forages were cutin, neutral detergent fiber, acid detergent fiber, acid detergent lignin, hemicellulose, cellulose, crude protein and starch. A. lampa showed the maximum degradation value and utilization of hemicellulose, and M. ephedroides and G. decorticans were the native forage species with the smallest value of degradation, in comparison with alfalfa hay. The correlation coefficient between acid detergent lignin and percentage of degradation and utilization of hemicellulose, suggests that lignin affects hemicellulolytic activity of P. xylanivorans. These results provide a framework to try to modify the activity of ruminal emicellulolytic environment by incorporating P. xylanivorans as probiotic strain.

Fil: Sosa Escudero, Miguel. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histologia y Embriología Mendoza. "Dr. Mario H. Burgos"

Fil: Arena, Graciela Nora. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Área Microbiología

Fil: Allegretti, Liliana Inés. Instituto Argentino de Investigaciones de las Zonas Áridas

Fil: Paez Lama, Sebastián. Instituto Argentino de Investigaciones de las Zonas Áridas

Fil: Egea, Vanina. Instituto Argentino de Investigaciones de las Zonas Áridas

Fil: Carcaño, Diego. Universidad Juan Agustín Maza (Mendoza, Argentina)

Fil: Grilli, Diego. Universidad Juan Agustín Maza (Mendoza, Argentina)

Country
Argentina
Keywords

Rumen, bacterias, Cultivo de cepas puras, HEMICELLULOSE, Hemicelulosa, Food processing and manufacture, GOATS, https://purl.org/becyt/ford/4.3, Degradación de la hemicelulosa, HEMICELULOSA, https://purl.org/becyt/ford/4, BACTERIAS, Pseudobutyrivibrio ruminis, Medios de cultivo, Cepas bacterianas, rumen, Pseudobutyrivibrio xylanivorans, S, cabras, Agriculture, TP368-456, RUMEN, CABRAS, BACTERIA, Cabras, Datos estadísticos, hemicelulosa, Bacterias

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
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