
handle: 11295/33702
Nucellar tissue cultures of polyembryonic Citrus sinensis cultivar Valencia Late and Citrus limon cultivar Rough Lemon produced somatic embryos within 60 days of culture on Murashige and Skoog (MS) basal medium supplemented with 0.4 g l-1 of filter-sterilised casein hydrolysate or 10% coconut water. Embryos that were separated and subcultured in MS media containing casein hydrolysate or coconut water but without hormones, first developed roots within 4 to 8 months and then shoots within 6 to 9 months of in vitro culture. Plantlets were transplanted to soil from 7 to 9 months after initiation. Callus-like tissue consisting of pseudobulbils was observed after 4 to 5 months from the time of initiation. Pseudobulbils developed into visible embryos with multiple cotyledons when subcultured into MS media containing casein hydrolysate or 10% coconut water after 19 to 27 days of in vitro culture. These embryos produced plantlets in which roots developed after 6 to 10 months and shoots formed after 8 to 11 months from the time of initiation. Key Words: Citrus, nucellar embryo culture, somatic embryogenesis (African Crop Science Journal: 2000, 8(2): 109-116)
Citrus, Citrus; nucellar embryo culture; somatic embryogenesis, nucellar embryo culture, somatic embryogenesis
Citrus, Citrus; nucellar embryo culture; somatic embryogenesis, nucellar embryo culture, somatic embryogenesis
| selected citations These citations are derived from selected sources. This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically). | 0 | |
| popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network. | Average | |
| influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically). | Average | |
| impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network. | Average |
