Tiller angle is one of important characters that constitute plant type with great significance in breading. A pair of japonica/indica lines, which were significantly different in tiller angle, were used in this study. A DH population were constructed via anther culture of F1 hybrids. The tiller angles of 115 DH lines were measured. QTL analysis was conducted based on the constructed molecular linkage map. Three QTLs (qTA-9a, qTA-9b and qTA-12) were detected on chromosome 9 and 12 respectively, Their variation were 22.7%, 11.9% and 20.9% respectively and their additive effects were all negative, showing this character was controlled by genes from big tiller angle parent ZYQ8. The application of the tiller angle that was controlled by major genes and minor genes in rice breeding is also discussed.