Listeria monocytogenes has been recognized as a bacteria that produces severe illness in animals and humans. Considering the importance of the presence of L. monocytogenes in soft paste cheeses, a study of diverse cheeses from supermarkets of direct sale to the public was carried out. From the 35 analyzed cheeses, 4 strains were isolated (11.4%). The result of the serological study showed that all the strains corresponded to the serotype 4. The proteic profiles of the isolated strains showed similarity with the used pattern (4b). Several authors reported the importance of L. monocytogenes as contaminant in foods ready to eat like the soft paste cheeses. Thus, it must be remarked the importance of the good handling practices in the production, transport, refrigeration and exhibition of this product.