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Electroforese e imunoquímica: I- A eletroforese das proteínas

Authors: FRANCO, António Pedro;

Electroforese e imunoquímica: I- A eletroforese das proteínas

Abstract

Tratamos neste trabalho dos fundamentos e métodos técnicos da electroforese, cujo interesse, como processo de investigação, parece desnecessário encarecer. Recordam-se, primeiramente, algumas noções relacionadas com a carga eléctrica da molécula proteica e comportamento desta num campo eléctrico. Descreve-se, a traços largos, a técnica, digamos, clássica, de electroforese em célula de vidro, analisando, com os sistemas de leitura, as características da separação obtida, assim como o significado das curvas. Trata-se por último, das técnicas de electroforese em papel de filtro, que analisam com maior pormenor, apontando-se as suas possibilidades e limitações.

This paper deals with the fundamentals and technical methods of electrophoresis, the interest of which, as a research process, seems unnecessary to emphasise. Firstly, we recall some notions related to the electrical charge of the protein molecule and its behaviour in an electrical field. The technique of electrophoresis in a glass cell is described in broad terms, analysing the characteristics of the separation obtained, as well as the significance of the curves. Finally, the filter paper electrophoresis techniques are analysed in greater detail, pointing out their possibilities and limitations.

Country
Portugal
Related Organizations
Keywords

Imunodiagnóstico, Análises clínicas, Biologia molecular

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
Green
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