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Biosensors and Bioelectronics
Article . 2007 . Peer-reviewed
License: Elsevier TDM
Data sources: Crossref
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Impedimetric immunosensor for the specific label free detection of ciprofloxacin antibiotic

Authors: Ionescu, Rodica Elena; Jaffrezic-Renault, Nicole; Bouffier, Laurent; Gondran, Chantal; Cosnier, Serge; Pinacho, Daniel; Marco, M.-Pilar; +3 Authors

Impedimetric immunosensor for the specific label free detection of ciprofloxacin antibiotic

Abstract

Impedance spectroscopy approaches combined with the immunosensor technology have been used for the determination of trace amounts of ciprofloxacin antibiotic belonging to the fluoroquinolone family. The sensor electrode was based on the immobilization of anti-ciprofloxacin antibodies by chemical binding onto a poly(pyrrole-NHS) film electrogenerated on a solid gold substrate. The electrode surface was modified by electropolymerization of pyrrole-NHS, antibody grafting and ciprofloxacin immunoreaction. The sensitive steps of surface modification, cyclic voltammetry (CV) and atomic force microscopy (AFM) imaging have been used for electrode surface characterization. The immunoreaction of ciprofloxacin on the grafted anti-ciprofloxacin antibody directly triggers a signal via impedance spectroscopy measurements which allows the detection of extremely low concentration of 10 pg/ml ciprofloxacin.

Country
France
Keywords

[SDE] Environmental Sciences, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SPI.OPTI] Engineering Sciences [physics]/Optics / Photonic, [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging, Polymers, [SDV]Life Sciences [q-bio], Impedance spectroscopy, Biosensing Techniques, [SPI.MAT] Engineering Sciences [physics]/Materials, Immunosensor, [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, Microscopy, Atomic Force, [SPI.MAT]Engineering Sciences [physics]/Materials, Ciprofloxacin, [SDV.IDA]Life Sciences [q-bio]/Food engineering, Electrochemistry, Immunoassay, [SDV.TOX.ECO] Life Sciences [q-bio]/Toxicology/Ecotoxicology, Molecular Structure, [SDE.IE]Environmental Sciences/Environmental Engineering, Polypyrrole, [CHIM.MATE]Chemical Sciences/Material chemistry, [SDV.IDA] Life Sciences [q-bio]/Food engineering, Molecular Biology/Molecular biology, Anti-Bacterial Agents, [CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry, [SDV] Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology, [SDE.IE] Environmental Sciences/Environmental Engineering, [CHIM.POLY] Chemical Sciences/Polymers, [CHIM.ANAL] Chemical Sciences/Analytical chemistry, [SPI.NANO] Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, [SDV.TOX.TCA]Life Sciences [q-bio]/Toxicology/Toxicology and food chain, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Pyrroles, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, [SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials, [CHIM.MATE] Chemical Sciences/Material chemistry, Reproducibility of Results, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, [SDV.IB.BIO] Life Sciences [q-bio]/Bioengineering/Biomaterials, [CHIM.THEO] Chemical Sciences/Theoretical and/or physical chemistry, [CHIM.POLY]Chemical Sciences/Polymers, [SDV.IB.IMA] Life Sciences [q-bio]/Bioengineering/Imaging, [SDV.TOX.TCA] Life Sciences [q-bio]/Toxicology/Toxicology and food chain, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, [SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
views
OpenAIRE UsageCountsViews provided by UsageCounts
82
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