An enhanced liquid chromatographic method for 5-hydroxymethylfurfural determination in UHT milk

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1992 Spain English Publisher:Springer Science and Business Media LLCJournal:Chromatographia, volume 33, pages 45-48 (issn: 0009-5893, eissn: 1612-1112,

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Authors: Morales, F. J.; Romero, C.; Jiménez Pérez, Salvio;

doi: 10.1007/bf02276850

handle: 10261/90285 , 10261/49364

An enhanced liquid chromatographic method for 5-hydroxymethylfurfural determination in UHT milk

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Abstract

An enhanced method of HPLC for the determination of 5-Hydroxymethyl-2-furfuraldehyde (HMF) in fluid milk was perfected. Its resolving capacity permitted the specific separation of this compound. The real concentration (μmol/l) of HMF was determined and not the HMF value, as in the colorimetric analysis, where an intermediary coloured compound (HMF-TBA) is utilised. The optimum pH condition (pH = 4.0-4.2) for the mobile phase to prevent overlapping between the HMF peak and the peaks of the other compound (α and β) that coeluted with the HMF and also the optimum organic solvent content (3% or 7% ) were established. This is a sensitive, quick and safe method for the determination of HMF in fluid milk.

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Spain

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Keywords

M ilk quality indicators, Heat-treated milk, 5-Hydroxymethyl-2-furfuraldehyde, Column liquid chromatography, Milk quality indicators, 5 hydortydroxymethyl-2-furfuraldehyde

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