A revalorization of discarded carrots as substrate for the production of second-generation ethanol is proposed. In order to increase the fermentable sugar concentration of the musts two strategies were studied: Strategy 1 consisted in the enzymatic hydrolysis of bagasse must and Strategy 2 by which carrots were milled, dropped into distilled water and hydrolyzed with different enzymes prior to compressing and filtering to obtain carrot must. By applying Strategy 2 using 0.05% (v/v) of the enzyme Optimase CX255 at 70°C and pH 5.5 during 2.5h, the fermentable sugars extracted increased 3.5 times. In this way, the production of 77.5L of ethanol for each ton of discarded carrots was achieved. This process yielded bagasse as byproduct, which could be used for animal feed.