Purification of angiotensin converting enzyme inhibitory peptides from sunflower protein hydrolysates by reverse-phase chromatography following affinity purification

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 2009 Spain English Publisher:Elsevier BVJournal:LWT - Food Science and Technology, volume 42, pages 228-232 (issn: 0023-6438,

Copyright policy )

Authors: Megías, Cristina; Pedroche, Justo; Yust, María del Mar; Alaíz Barragán, Manuel; Girón-Calle, Julio; Millán, Francisco; Vioque, Javier;

doi: 10.1016/j.lwt.2008.05.003

handle: 10261/55330

Purification of angiotensin converting enzyme inhibitory peptides from sunflower protein hydrolysates by reverse-phase chromatography following affinity purification

- Summary
- Subjects
- Metrics

Abstract

The purification of a peptidic fraction with angiotensin converting enzyme (ACE) inhibitory activity from sunflower protein hydrolysates by affinity chromatography was recently described. We now describe that reverse-phase HPLC fractionation of this product yields several fractions with IC50 one order of magnitude higher than those previously purified by reverse-phase HPLC following gel filtration chromatography, showing that affinity chromatography is much more effective than gel filtration chromatography as a first step for purification of ACE inhibitory peptides. The amino acid composition of these fractions is presented, but attempts to determine their amino acid sequence failed, showing that these fractions contained more than one peptide.

This work was supported by research grants AGL 2004-03930 (F.M.) and AGL 2005-01120 (J.G.-C.) from the Spanish Ministry of Education and Science, partially supported by FEDER funds from EU.

Peer reviewed

Country

Spain

Related Organizations

Instituto de la Grasa
Spain
Spanish National Research Council
Spain

Keywords

Inhibitory peptides, Affinity purification, Angiotensin converting enzyme, Sunflower protein hydrolysate

Impact byBIP!

	selected citations These citations are derived from selected sources. This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	38
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Top 10%
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Top 10%
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Top 10%