SummaryBackgroundEosinophils are prominent effectors of allergic inflammation. Taurine‐chloramine (TauCl), a derivative of the amino acid taurine, shows antioxidant properties in different cell systems but its effects on eosinophils have not been reported.ObjectiveTo study the effects of TauCl and taurine on functional responses of isolated human eosinophils activated by different stimuli.MethodsHuman eosinophils were purified from the blood of healthy donors by a magnetic bead separation system. The effects of TauCl and taurine (0.1–1 mm) were investigated on the generation of superoxide anion (ferricytochrome‐creduction microassay), calcium signal (fluorimetry), p47phox–p67phoxtranslocation (Western blot), leukotriene C4(LTC4) production (enzymeimmunoassay), eosinophil peroxidase (EPO) release (spectrophotometry), eosinophil cationic protein (ECP) release (radioimmunoassay), apoptosis (flow cytometry with annexin V‐propidium iodide), and nuclear factor‐κB (NF‐κB) activation (Western blot).ResultsTauCl inhibited superoxide anion generation triggered byN‐formyl‐Met–Leu–Phe (fMLP; 30 nm), phorbol myristate acetate (1 nm) and serum opsonized zymosan (0.5 mg/mL) with similar potency (IC50∼200 μm) for the three stimuli, while taurine (0.1–1 mm) was scarcely effective. TauCl but not taurine inhibited p47phox–p67phoxtranslocation. TauCl (200 μm) and taurine (1 mm) did not modify the [Ca2+]iresponses to fMLP. TauCl inhibited the release of EPO (IC50∼200 μm) and reduced ECP and LTC4production from fMLP‐activated eosinophils while taurine was without significant effects. TauCl (1 mm) did not change constitutive apoptosis but significantly attenuated the ability of granulocyte‐monocyte colony‐stimulating factor (GM‐CSF) and IL‐5 to prevent apoptosis. The activation of eosinophil NF‐κB induced by GM‐CSF and IL‐5 was suppressed by TauCl.ConclusionTaurine is without significantin vitroeffects on human eosinophil functions but its derivative TauCl inhibits oxidative burst and generation of inflammatory mediators, and reverses the survival effect produced by inflammatory cytokines. Therefore, endogenous TauCl may help to suppress excessive inflammatory response in eosinophils at inflammatory sites.