Accurate information on the spatial distribution of weeds is the key to effective site-specific weed management and the efficient and sustainable use of weed control measures. This work focuses on the early detection of johnsongrass, common cocklebur and velvetleaf present in a corn field using high resolution airborne hyperspectral imagery acquired when corn plants were in a four to six leaf growth stage. Following the appropriate radiometric and geometric corrections, two supervised classification techniques, such as spectral angle mapper (SAM) and spectral mixture analysis (SMA) were applied. Two different procedures were compared for endmember selections: field spectral measurements and automatic methods to identify pure pixels in the image. Maps for both, overall weeds and for each of the three weed species, were obtained with the different classification methods and endmember sources. The best results were achieved by defining the endmembers through spectral information collected with a field spectroradiometer. Overall accuracies ranged between 60% and 80% using SAM for maps that do not differentiate the weed species while it decreased to 52% when the three weed species were individually classified. In this case, the SMA classification technique clearly improved the SAM results. The proposed methodology shows it to be a promising prospect to be applicable to low cost images acquired by the new generation of hyperspectral sensors onboard unmanned aerial vehicles (UAVs).