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Quantitative estimates are important to establish whether pork adulteration in ground beef and pâté is accidental or intentional. A PCR procedure has been developed and evaluated to quantify pork in heated and nonheated meat and pâtés by densitometry using a specific and sensitive repetitive DNA element. Thirty, twenty-five, and twenty PCR cycles were carried out to find the best standard curve and correlation between pork content and band intensity. Twenty cycles showed the best results, quantifying degree contamination up to 1% pork in beef (heated and nonheated) and pork in duck pâté with a minimum error. Finally, fraud was found in commercial pâtés.
Pig, Meat, Swine, Fraud, Food Contamination, DNA, Polymerase Chain Reaction, Meat Products, Contamination, Quantification, Animals, Cattle, Species identification, Short Interspersed Nucleotide Elements
Pig, Meat, Swine, Fraud, Food Contamination, DNA, Polymerase Chain Reaction, Meat Products, Contamination, Quantification, Animals, Cattle, Species identification, Short Interspersed Nucleotide Elements
| selected citations These citations are derived from selected sources. This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically). | 69 | |
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| influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically). | Top 10% | |
| impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network. | Top 10% |
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