Application of Reproductive Biotechnology for the Recovery of Endangered Breeds: Birth of the First Calf of Murciana–Levantina Bovine Breed Derived by OPU, In vitro Production and Embryo Vitrification

descriptionPublicationkeyboard_double_arrow_right Article 21 May 2013 English Publisher:WileyJournal:Reproduction in Domestic Animals, volume 48 (issn: 0936-6768, eissn: 1439-0531,

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Authors: Ruiz, S.; Romero-Aguirregomezcorta, J.; Astiz, S.; Peinado, B.; Almela, L.; Poto, A.;

doi: 10.1111/rda.12179

pmid: 23692028

handle: 10261/291802

Application of Reproductive Biotechnology for the Recovery of Endangered Breeds: Birth of the First Calf of Murciana–Levantina Bovine Breed Derived by OPU, In vitro Production and Embryo Vitrification

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Abstract

ContentsIn a conservation project, reproductive biotechnology was implemented for the recovery and conservation of an endangered bovine breed in Spain. The breed Murciana–Levantina, declared to be worthy of special protection status (http://www.fao.org/newsroom/en/news/2006/1000464/index.html), is of great interest because of its hardness, longevity, docility and disease resistance. This contribution describes the birth of the first calf of this breed obtained by reproductive biotechnology, using ultrasound‐guided punction and aspiration of ovarian follicles, in vitro embryo production, vitrification of embryos by a cryotop device and, finally, the transfer of cryopreserved embryos to recipient heifers of a commercial dairy herd.

Related Organizations

University of Murcia
Spain

Keywords

Conservation of Natural Resources, Oocyte Retrieval, Fertilization in Vitro, Breeding, Embryo Transfer, In Vitro Oocyte Maturation Techniques, Embryo Culture Techniques, Pregnancy, Oocytes, Animals, Cattle, Female

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