Abstract Aims The aim of this work was to evaluate the fertilizing effect of three anaerobic co-digestates on the growth of the herbaceous plant Lolium rigidum. Methods Nine treatments, combining different nutritional solutions (organic and inorganic) and number of fertilizations (one or two) were evaluated. Organic nutritive solution: plants grown with different olive mill solid waste (OMSW) -microalgae co-digestates: 75% OMSW-25% Raphidocelis subcapitata, volatile solids (VS) basis (OMSW-Rs); 50% OMSW- 50% Chlamydomonas Reinhardtii, VS basis (OMSW-Chl); and 75% OMSW-25% Secenedesmus quadricauda, VS basis (OMSW-Sq). Inorganic nutritive solution (INS): plants grown with inorganic Hoagland nutrient solution at 50%. After 60 days of experimentation, biometric and nutritional characteristics and photosynthetic activity were measured. Results The results showed a favourable growth, development and nutritional quality of L. rigidum plants when digestates obtained from the anaerobic co-digestion of OMSW-microalgae are used as organic nutritional solutions as opposed to INS ones. The highest total biomass of L. rigidum was obtained with the treatments that involved two fertilizations. No inhibition due to excess nutrients was observed. A higher root/shoot ratio was achieved with the digestates of OMSW-Rs and OMSW-Ch as compared to that obtained with OMSW-Sq (F = 17.23 p ≤ 0.001). The nitrogen shoot biomass obtained after the organic treatments with the above-mentioned co-digestates was higher than that obtained after the inorganic treatment. Net photosynthesis rates did not present differences in the co-digestates treatments, being equal or superior to the INS treatments. Conclusions The use of the anaerobic co-digestates from OMSW-microalgae can be considered a viable and promising alternative to inorganic fertilization.