The work has been supported by the Spanish Ministry of Economy and Competitiveness of Spanish government through the Research Project AGL2016-80231-R. This work is part of the doctoral thesis of Zaira Pardo, Ph.D. student from the Official Doctoral Program ‘‘Nutrition and Food Sciences” of the Granada University, who received a grant FPI with reference BES-2017-081486. Heat stress (HS) lead to intestinal epithelial damage through induction of cellular oxidative stress. Since Zn is an essential nutrient with antioxidant properties that is involved in maintaining the intestinal barrier function, this study aimed to examine the effects of Zn supplementation in the intestinal epithelium under HS. Caco-2 cells were pre-incubated with Zn (50 and 100 µM) prior to control (37 °C) or HS conditions (41–42 °C) for 24 h. Zn pretreatment reduced the lactate dehydrogenase release, a parameter reflecting the cell membrane damage. Zn supplementation alleviated the ROS generation in cells exposed to HS and promoted the activity of antioxidant enzymes. Moreover, Zn (specially 50 µM) partly attenuated the HS induced damage on monolayer integrity as measured by transepithelial electrical resistance and phenol red permeability. Therefore, Zn supplementation had beneficial effects on the intestinal damage evoked by high temperatures and may be a useful nutritional strategy against global warming. The work has been supported by the Spanish Ministry of Economy and Competitiveness of Spanish government through the Research Project AGL2016-80231-R. This work is part of the doctoral thesis of Zaira Pardo, Ph.D. student from the Official Doctoral Program ‘‘Nutrition and Food Sciences” of the Granada University, who received a grant FPI with reference BES-2017-081486.