Editors: Laura Nuño de la Rosa, Gerd B. Müller. This chapter introduces the reader to anatomical network analysis (AnNA): a conceptual framework for the tolopological analysis of organismal form. AnNA focuses on the structural relations among anatomical parts, which allows for an evaluation of morphological organization in comparative analyses for both development and evolution. The nodes of the network represent anatomical elements, and the links that connect them represent structural relations or interactions among these elements. Network theory provides the methods to analyze these anatomical network models. The first and second sections present the historical and conceptual background of this framework. The third section explains the construction of anatomical networks and some of the basic parameters we can use to characterize the topology of these models and infer their morphological organization. The fourth section summarizes the interpretation of network parameters in terms of morphological complexity, hierarchy, integration, and modularity in the context of morphological evo-devo. The fifth section introduces the classical construction rules to build null models for networks and an example of the use of network null models in morphology. Finally, in the sixth section, we have explored some of the limits of AnNA. BE-A is funded by a European Union’s Horizon 2020 Marie Skłodowska-Curie Grant No. 654155. DRG is funded by MINECO-FEDER BFU2015-70927-R.