In this paper, the most recent applications of high-performance size-exclusion chromatography (HPSEC) in analyses of fats and oils are presented. Directly used in oils, the technique shows utility for evaluation of polymers in used frying fats and oxidized samples, as well as for analysis of low-caloric fats in blends. In concentrated fractions of polar compounds, characterization of virgin and refined oils and follow-up of early oxidation stages are also attainable. An extended application to fatty acid derivatives allows complementary analyses of specifically oxidized and polymeric fatty acyl groups.

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