
handle: 10261/235354
[EN] Morphological and molecular approaches to the study of ophiuroids (Echinodermata: Ophiuroidea) at Las Baulas Marine National Park (North Pacific, Costa Rica). [Introduction]: Information on the class Ophiuroidea is very scarce, despite it consisting of the highest number of species within the phylum Echinodermata, with up to 2 100 species. In particular, there are hardly any study focused specifically to ophiuroids found along the Pacific coast of Costa Rica. Objective: Morphological and genetic characterization of ophiuroids of the shallow intertidal and sublittoral areas of Las Baulas Marine National Park (Pacific coast of Costa Rica). [Methods]: Sampling was conducted between January and April of 2018 at seven locations: Punta del Morro, Playa Carbón, Playa Ventanas, Playa Grande, Playa Tamarindo Ciudad, Playa Tamarindo and Playa Langosta. A total of 214 ophiuroids were characterized using taxonomic keys and original descriptions and a molecular analysis of the mitochondrial Cytochrome oxidase I (COI) gene. [Results]: An analysis of morphological and genetic characters indicates that 11 species are present in the study site. Four may represent new species, nine constitute new records for the area and one (Ophiophragmus aff. stellatus) is recorded in Costa Rica for the first time. Additionally, juvenile individuals of different species were identified, and two cryptic species were detected. [Conclusions]: According to the molecular analysis, COI is useful to delimit ophiuroid species and to establish phylogenetic relationships at lower (genera and species) but not higher taxonomic levels. This study also provides images and morphological remarks for future comparative studies.
[ES] [Introducción]: El conocimiento de la Clase Ophiu-roidea es muy escaso y deficiente, a pesar de ser la que presenta el mayor número de especies del Filo Echinodermata con hasta 2100. Además, apenas existen publicaciones dedicadas específicamente al grupo de las ofiuras en la vertiente pacífica de Costa Rica. [Objetivo]: La caracterización morfológica y genética de las ofiuras de la franja intermareal y sublitoral somero del Parque Nacional Marino Las Baulas (Pacífico de Costa Rica). [Métodos]: El periodo de recolecta se extendió entre enero y abril de 2018 y tuvo lugar a lo largo de siete localidades: Punta del Morro, Playa Carbón, Playa Ventanas, Playa Grande, Playa Tamarindo Ciudad, Playa Tamarindo y Playa Langosta. Se ha realizado la caracterización de 214 ofiuras mediante claves taxonómicas, descripciones originales y el marca-dor mitocondrial citocromo c oxidasa subunidad I (COI). [Resultados]: En total, combinando los caracteres morfoló-gicos y genéticos, se han identificado 11 especies, cuatro de las cuales podrían ser nuevas, nueve constituyen nuevos registros en el parque y una de las especies se ha encon-trado por primera vez en Costa Rica (Ophiophragmus aff. stellatus). Además, se han identificado individuos juveniles y se han encontrado dos especies crípticas. [Conclusión]: Del estudio molecular se concluye que el marcador COI es útil para delimitar molecularmente las especies y establecer relaciones filogenéticas para los niveles taxonómicos de género y especie, pero no para los superiores. Se aportan observaciones morfológicas e imágenes como contribución a la taxonomía del grupo
Este estudio ha sido parcialmente cubierto por el Ministerio de Economía y Competitividad (proyecto CTM2014-57949-R).
Ophiuroids, Eastern Tropical Pacific, barcode, molecular identification, Molecular identification, ophiuroids, Echinodermata, Barcode
Ophiuroids, Eastern Tropical Pacific, barcode, molecular identification, Molecular identification, ophiuroids, Echinodermata, Barcode
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