Phosphoglucomutase, phosphoglucose isomerase, and glutamate oxaloacetate transaminase isozymes have been studied in 'Chinese Spring' – 'Imperial', 'Holdfast' – 'King II', and 'Kharkov'–'Dakold' wheat–rye addition lines using starch and polyacrylamide gel electrophoresis. The analyses were conducted with different parts of dry kernels (embryo plus scutellum, and endosperm), leaves, and roots. In all cases the zymogram phenotypes obtained were the same, independent of the tissue analysed. Germination and maturation processes were also studied. One locus involved in the production of phosphoglucomutase isozymes was located on chromosome 4R of 'Dakold' and on 4RS of 'Imperial' and 'King II' cultivars. Evidence was obtained that one locus controlling the phosphoglucose isomerase isozymes is located on chromosome 1R of these three rye cultivars analysed, specifically on the short arm of chromosome 1R in 'King II'. Also, three loci controlling the glutamate oxaloacetate transaminase isozymes have been placed on chromosomes 7R, 6R, and 3R of the rye cultivars analysed, and more exactly, on 7RL of 'Imperial' and 'King II' and on 6RL of 'King II'. Biochemical evidence of homology between chromosome arms of the different cultivars of rye analysed, and homoeology between chromosome arms of wheat and rye is reported. Germination and maturation processes support the hypothesis that the expression of rye isozymes studied in dry kernel, leaf, and root, and during germination and maturation, is controlled by the same chromosome arms.Key words: wheat–rye addition lines, isozyme structural genes, phosphoglucomutase, phosphoglucose isomerase, glutamate oxaloacetate transaminase.