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Application of molecular nucleic acid-based techniques for the study of microbial communities in monuments and artworks.

Authors: González, Juan M.; Saiz-Jiménez, Cesáreo;

Application of molecular nucleic acid-based techniques for the study of microbial communities in monuments and artworks.

Abstract

Los microorganismos desempeñan papeles críticos en todo tipo de hábitat de la Tierra, incluso en los que han sido construidos por los humanos. Así, el patrimonio cultural también se ve afectado por la colonización de microorganismos. Mientras los métodos clásicos de microbiología basados en procedimientos de cultivo han proporcionado información importante, pero limitada, de las muestras naturales, las nuevas técnicas moleculares son muy valiosas para descubrir la diversidad de la microbiota causante del biodeterioro de monumentos y obras de arte. El conocimiento alcanzado con estos métodos ha permitido diseñar estrategias adecuadas para la conservación y protección de monumentos, en beneficio de generaciones futuras. Esta revisión describe la situación actual de la aplicación de métodos moleculares al análisis de bienes culturales y aporta perspectivas para un futuro próximo.

Microorganisms play critical roles in every kind of habitat on Earth, including those constructed by humans. Thus, our cultural heritage is affected by microbial colonization. While classical microbiological methods based on culturing procedures have provided important, but limited information on the microbial diversity of natural samples, novel molecular techniques have been extremely valuable in unraveling the diversity of microbiota involved in the biodeterioration of our monuments and artworks. The knowledge gained from these approaches has allowed the design of strategies for conserving and protecting monuments for the benefit of future generations. This review describes the state-of-the-art of the application of molecular methods to the analysis of cultural assets, and provides nearfuture perspectives on the subject.

JMG received a Spanish Ministry of Education and Science (MEC) contract from the ‘Ramón y Cajal’ program. This study was partially supported by a MEC project BTE2002-04492-C02-01.

Peer reviewed

Related Organizations
Keywords

DNA, Bacterial, técnicas moleculares, Bacteria, microbial communities, Microbial communities, Sculpture, Cultural Heritage, cultural heritage, RNA, Bacterial, Biodegradation, Environmental, Genetic Techniques, Molecular techniques, patrimonio cultural, Paintings, comunidades microbianas, molecular techniques, Ecosystem

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    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    Average
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
30
Average
Top 10%
Top 10%
Green