AbstractCarbon black nanoparticle (CBNP) press‐transferred film‐based transducers for the molecular detection at the microscale level were proposed for the first time. Current‐sensing atomic force microscopy (CS‐AFM) revealed that the CBNP films were effectively press‐transferred, retaining their good conductivity. A significant correlation between the morphology and the resistance was observed. The highest resistance was localized at the top of the press‐transferred film protrusions, whereas low values are usually obtained at the deep crevices or grooves. The amount of press‐transferred CBNPs is the key parameter to obtain films with improved conductivity, which is in good agreement with the electrochemical response. In addition, the conductivity of such optimum films was not only Ohmic; in fact, tunneling/hopping contributions were observed, as assessed by CS‐AFM. The CBNP films acted as exclusive electrochemical transducers as evidenced by using two classes of molecules, that is, neurotransmitters and environmental organic contaminants. These results revealed the potential of these CBNP press‐transferred films for providing new options in microfluidics and other related micro‐ and nanochemistry applications.