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Journal of Agricultural and Food Chemistry
Article . 2000 . Peer-reviewed
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Flavonoids and Phenolic Acids of Sage: Influence of Some Agricultural Factors

Authors: Areias, Filipe M.; Valentão, Patricia; Andrade, Paula B.; Ferreres, Federico; Seabra, Rosa M.;

Flavonoids and Phenolic Acids of Sage: Influence of Some Agricultural Factors

Abstract

A reversed-phase high-performance liquid chromatography/diode-array detector procedure is proposed for the determination of six phenolic compounds (caffeic acid, luteolin 7-O-glucoside, rosmarinic acid, apigenin, hispidulin, and cirsimaritin) in sage. The chromatographic separation was achieved using a reversed-phase Spherisorb ODS2 (5-microm particle size, 25.0 x 0.46 cm) column. Of the several extractive solvents assayed only ethyl ether, ethyl acetate, and acetone were able to extract all the compounds mentioned. Best resolution was obtained using a gradient of water/phosphoric acid (999:1) and acetonitrile. Ten samples cultivated in two experimental fields (1997-1999) were analyzed and the individual compounds quantified. Four commercially available samples were also analyzed and the results are discussed.

Keywords

Flavonoids, Phenolic profile, Lamiaceae, Reverse Transcriptase Polymerase Chain Reaction, Hydroxybenzoates, Quality control, Sage (Salvia officinalis), HPLC/DAD

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
views
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71
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