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handle: 10261/162459
[ES] Se describe un método bioquímico, basado en el análisis y comparación de proteínas totales y de determinados isoenzimas (fosfatasas ácidas, fosfatasas alcalinas y peroxidasas). que permite conocer si va a existir o no compatibilidad entre un injerto y un portainjerto dados sin que para ello sea necesario realizar el injerto entre ambos. Este método -puesto a punto en los laboratorios de la Misión Biolóxica de Galicia— parece indicar que siempre que exista una elevada similitud proteica entre injerto y patrón va a existir una buena afinidad al injerto entre ambos, y que, por el contrario, la incompatibilidad será manifiesta cuando los componentes proteicos anteriormente citados sean muy diferentes. Se presentan los valores de los coeficientes de afinidad entre injerto y patrón (Ki_p) y entre patrón e injerto (Kp_i) obtenidos para las diferentes combinaciones de los cultivares ALBARIÑO, CAIÑO y PALOMINO FINO con seis portainjertos de utilidad en nuestro medio vitícola (420—A, 41-B, 99-R, 110-R, 161-49, 196-17C). De ellos se puede deducir que el ALBARIÑO ha de ser compatible con los portainjertos 110-R, 41-B y 161-49; el CAIÑO con el 110-R, el 196-17C y el 161-49 y el PALOMINO FINO con el 41-B. Estos resultados coinciden con las prácticas habituales de los agricultores gallegos.
[EN] A biochemical method for the determination of the scion—rootstock affinity or incompatibility in grape is described. With the application of this method it is possible to know "a priori” which rootstocks are compatible with a given scion. Total proteins, acid phosphatases, alkaline phosphatases and peroxidases of three Vitis vinifera cultivars (ALBARIÑO, CAIÑO, PALOMINO FINO) and six stocks (420—A, 41-B, 99-R, 110-R, 161-49, 196-17C) have been extracted and analysed by polyacrilamide gel electrophoresis. From the relative electrophoretic mobilities (REM) of total proteins, the affinity indexes between cultivars and stocks (Ki_p) and stocks and cultivars (Kp_i) combinations were calculated. With this results we can concluded that the cultivar ALBARIÑO should be compatible with the 110-R, 4l-B and 161-49 roots-stocks; CAIÑO with the 110-R, 41-B and 161-49, and PALOMINO FINO with 41-B. This results are in agreement with farmers' cultural practices.
Comunicación presentada en el I Congresso Ibérico de ciências hortícolas, celebrado en Lisboa entre el 18 y el 21 de junio de 1990.- 6 páginas, 1 tabla y 1 figura.
Peer reviewed
Scion-stock affinity, Vitis vinifera, Compatibilidad patrón-injerto, Galicia
Scion-stock affinity, Vitis vinifera, Compatibilidad patrón-injerto, Galicia
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