The content and composition of lipids isolated from the seeds of seven apple species from Asturias (Spain) were characterized. The highest content in oil corresponded to Collao (22.73±0.81 g·100 g-1 of seed), followed by Raxao and Riega (20.19±0.74 g·100 g-1 of seed; 19.67±0.85 g·100 g-1 of seed), respectively. The linoleic acid was found to be the main component in Limón Montés (60.78±3.07%) followed by Riega (60.01±3.41%). Solarina seed oil was the one with the highest content in total sterols (558.52±9.42 mg·100 g-1 of oil) while Blanquina was the one that presents the lowest amount (166.55±1.89 mg·100 g-1 of oil). Phosphatidylcholine (70.58±3.85%) was found to be the main constituent in Blanquina followed by Collaos (55.55±2.96%). Raxao presented the highest content in β-tocopherol (125.29±12.62) and α-tocopherol was the most important tocopherol in Limón Montés (84.68±5.61 mg·kg-1 of oil). The main triglycerides were LLP (41.17±1.98-39.32±1.66%) followed by LLL (27.12±1.32−17.80±1.96%). Good separation among specie samples according to the statistical analysis of the principal component (PCs) and linear discriminant analysis (LDA) was obtained.