Purpose: To find out the bacteria involved in chronic dacryocystitis and their sensitivity to different antibiotics. Materials and methods: Study conducted on 200 patients. Inclusion criteria for the study are 1)Watering/mucopurulent discharge from eyes for more than 3 months.2)Preoperative evalution for cataract surgery revealing chronic dacryocystitis, excluding patients with block of nasolacrimal duct due to tumors and drugs. Discharge from puncta was collected with a moistened sterile swab without touching the lid margin or adjacent skin. In cases, without discharge, specimen was collected from the fluid regurgitating from puncta on lacrimal syringing. Material was sent for Gram’s staining and culture plating on blood agar, Mc Conkey’s agar. Examination of the plates was done after 18-24 hours. If no growth is seen, plates are further incubated for 48 hours. Sensitivity to antibiotics was tested by Kirky Bauer method or Muller-Hinton agar method. Results: Out of 200 patients, 115 showed a positive bacterial growth, of which 74% were gram-positive and 34% were gram-negative organisms, 85 did not show any growth. Staphylococcus aureus 37, Klebsiella 17, Pneumococci 13, mixed cultures7, Staphylococcus epidermidis 10, alpha hemolytic streptococci 5, B. catarrhalis 5 and anaerobes 3. The antibiotic sensitivity patterns are as below , Staphylococcus aureus sensitive to Ciprofloxacin, Klebsiella sensitive to Gentamicin, Ciprofloxacin, Norfloxacin and Cotrimoxazole, Pneumococci sensitive to Amoxycillin, Penicillin, E.coli sensitive to Gentamicin, Ciprofloxacin and Norfloxacin and B. catarrhalis sensitive to Amoxycillin, Penicillin, Ciprofloxacin, Norfloxacin and Doxycycline. Conclusions: The present study revealed that most commonly encountered organism was Staphylococcus aureus which is most sensitive to Ciprofloxacin.