Aphis gossypii Glover is a highly polyphagous pest that causes economic losses to many crops worldwide. Evolution of resistance to insecticides is a major concern when managing populations of A. gossypii, and resistance has been reported in numerous studies. In the southeastern U.S., few effective insecticides have historically been available for management of A. gossypii, and a reduction in efficacy of imidacloprid, one of the most widely used active ingredients, has been observed by stakeholders in Alabama. The objective of this project was to quantify the susceptibility of A. gossypii populations to imidacloprid. The LC50s at 72 h for field collected colonies, ranged from 1.11 – 19.23 ppm, while resistance ratios ranged from 35.11 – 607.16. The results from this study confirmed imidacloprid resistance in A. gossypii populations across Alabama cotton production regions.