Antrodia juniperina is one of the most common Antrodia genus species in Turkey, which is regarded as a medicinal plant in some countries. The aim of this study is to determine the volatile aroma compounds (compounds) of A. juniperina. The volatile aroma compounds of A. juniperina were analyzed by headspace gas chromatography (GC-MS). 22 volatile compounds such as alcohols, aldehyde, acids, ketones, terpenes, ester, 11,13-dihydroxy-11.β.H-arbusculin benzofuran, 2H-1-benzopyran, 6-α-cadina-4,9-diene, and diethyl phthalate were determined. Nerolidol (alcohol), 11,13-dihydroxy-11.β.H-arbusculin benzofuran, 3-methoxy-5-methyl-benzaldehyde (aldehyde), 3-octanone (ketone), 3,7-dimethyl-1,6-octadien-3-ol (alcohol), and 3-octanol (alcohol) were identified as major aroma components in A. juniperina with 76.22%. Key words: Nerolidol, 3-Octanol, headspace, gas chromatography-mass spectrometry.