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African Journal of Biotechnology
Article . 2012 . Peer-reviewed
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https://dx.doi.org/10.5897/ajb...
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GFLV coat protein constructs based on local isolates from the Czech Republic

descriptionPublicationkeyboard_double_arrow_right Article 16 Jan 2012Publisher:Academic JournalsJournal:AFRICAN JOURNAL OF BIOTECHNOLOGY, volume 11 (eissn: 1684-5315, Copyright policy )
Authors: null Josef Vlasák;

doi: 10.5897/ajb11.2805

GFLV coat protein constructs based on local isolates from the Czech Republic

Abstract

Coat protein genes of grapevine fanleaf virus local strains isolated in South-Moravia, Czech Republic were sequenced, and artificial coat protein gene was designed and synthesized. It retains typical sequence features of local strains but is free of mRNA destabilizing sequences. Three variants of the synthetic gene were cloned intoAgrobacterium plant expression vector and their function was tested after potato protoplasts transformation, assaying transient mRNA and coat protein production. Key words: Grapevine fanleaf virus, Vitis vinifera, sequence, protoplast expression, resistance.

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
gold