Wild Huperzia serrata was transplanted to moss, sand, corrosion soil, experimental garden soil, vermiculite mixed with perlite (1:1), and corrosion soil mixed with garden soil (1:1). The highest survival rate (90.0%) was observed in the moss medium, the second highest rate (53.3%) was detected in corrosion soil medium, and the lowest rate was found in garden soil medium. The physiological indices of H. serrata in response to the six media were also determined. The dry/wet ratio of H. serrata was inversely correlated with the survival rate across the different media, whereas the chlorophyll content and root activity of the species were positively correlated with it. The findings suggest that moss is the optimal medium for the transplantation of H. serrata. Key words: Huperzia serrata, media, transplantation.