Tobacco Rattle Virus is a plant-borne virus of the genus Tobravirus, which has a wide range of host species. Its unique characteristics and versatility have made it a highly sought-after tool in plant sciences and biotechnology. TRV is characterized by its bipartite genome structure, which is composed of two RNA1s and two RNA2s, allowing for the efficient insertion of foreign genes into RNA2 for gene delivery. Additionally, TRV is associated with the viral gene silencing phenomenon (VIGS), which involves the insertion of a fragment of the desired gene into the virus genome. This has become a useful tool in the study of gene function and understanding of plant-microbe interactions. Finally, TRV is discussed in relation to its RNA interference-mediated resistance (RNAi) against plant pathogens, which is induced by the expression of short interfering RNA molecules (siRNAs). In this chapter, TRV is presented as a vector with numerous potential benefits, however, it is also discussed that there are challenges and limitations to its use. For example, due to the narrow host range, TRV is only applicable to certain plant species. Therefore, efforts must be made to expand the host range or to develop alternative vectors. Additionally, optimizing viral constructs, delivery techniques, and purification methods is essential for increasing the efficiency and scaling of TRV-induced gene expression and gene suppression. In conclusion, TRV is a valuable tool with the potential to transform plant biotechnology and promote sustainable agriculture practices.