The objective of this study was to determine the resistance of Cronobacter sakazakii in acidic environments. The D-valuesof CAFM2 (ATCC 29544), EB 1, EB 5, and EB 41 at pH 2.5 in TSB were significantly (p<0.05) higher when cells wereadapted at pH 4.5 in TSB for 5-h then when cells were not adapted at pH 4.5 in TSB. The D-values of CAFM2, EB1, andEB 41 at pH 2.5 in TSB were significantly (p<0.05) higher when cells were adapted at pH 4.5 in TSB for 10-h then whencells were not adapted at pH 4.5 in TSB. The D-values of CAFM2 and EB1 at pH 2.5 in TSB were significantly (p<0.05)higher when cells were adapted at pH 4.5 in TSB for 24-h then when cells were not adapted at pH 4.5 in TSB. The adaptationof C. sakazakii to mild acidic environments may result in increased resistance to severe acidic environments. The D-valuesof all test strains at pH 2.5 in TSB were significantly (p<0.05) higher when cells were cultured at pH 4.5 then when theywere cultured at pH 7.2 in TSB. These data indicate that cells cultured in mildly acidic environments may result in increasedresistance to severe acidic environments. The acid adaptation of C. sakazakii showed an increased resistance to acidic envi-ronments. The acid adaptation response of C. sakazakii has important implications for food safety, which should be consid-ered when food preservation measures are developed.Key words: Cronobacter sakazakii, acid environment, response of acid adaptation, acid resistance