Brassinolide (BL) is a plant hormone showing wide occurrence in the plant kingdom with unique biological effects on growth and physiological traits. The fig varieties, Improved Brown Turkey (IBT) and Masui Dauphine (MD), are commonly found in Indonesia and Malaysia. There is limited information on exogenous brassinolide application on these varieties. In this chapter, we present the effect of different concentration of exogenous application of BL on growth and physiological changes of fig. Increasing BL concentration (50, 100, and 200 ml.L−1) caused some differences in growth and physiological changes of fig, but the differences were not consistent and most of the changes happened only in first or second month. Cultivar IBT showed higher growth and physiological changes than cultivar MD after receiving brassinolide treatment. There was significant effect of interaction between brassinolide and variety on growth and physiological changes of fig except in plant height and total dry biomass.