Strawberry is a widely grown hybrid species of single ovary fruit that is indehiscent and the scientific name of strawberry is Fragaria ananassa belongs to the family Rosaceae which is native to America, Europe, Asia and cultivated worldwide for its fruits. The fruits are rich in vitamin C. The phytochemical constituents such as gallotannin, ellagitannin, ellagic acid anthocyanins, proanthocyanins, catechin, cyanidin, agrimonium, coumaroyl glycosides, pedunculagin, pelargonidin, flavonols, chlorogenic acid, salicylic acid, caffeic acid, and chlorogenic acid have various medicinal benefits like regulating heart function, antidiarrheal, antioxidant, diuretic, decline blood pressure, elevate good cholesterol, esophageal cancer, etc. This chapter deals with nutritional value, phytoconstituents, and its structure along with medicinal uses of Strawberry.