Nitrogen (N) is a major essential element for all organisms, and generally the amount of available N (mainly inorganic nitrogen such as nitrate or ammonia) in soil is limiting factor for natural and agricultural plant production [40]. Biological nitrogen fixation (BNF) is a process by which atmospheric dinitrogen (N2) is reduced into 2 molecules of ammonia (NH3) by the enzyme nitrogenase with 8H+, 8eand 16 Mg ATP. BNF have important role in N cycle in both global ecosystem and agro-ecosystem. Based on the data compiled by Bezdicek and Kennedy in 1988 [11], about 175 million metric tons of nitrogen per year is estimated to be fixed in global ecosystems, in which 90 million metric tones in agricultural land, 50 million metric tones in forest and non-agricultural land, and 35 million metric tones in sea. At that time, nonbiological nitrogen fixation was estimated about 50 million metric tones per year by industrial nitrogen fixation mainly for the synthesis of ammonia fertilizer, and about 20 million metric tones by combustion, and about 10 million metric tones by lightening. In 2009, the production of N fertilizers increased to 106 million metric tones (FAOSTAT), but the amount of BNF still exceeds over non-biological nitrogen fixation.