In recent years, two problems roused peoples’ concern. One is energy crisis caused by the depleting of petroleum fuel. The other is environmental issues such as greenhouse effect, global warming, etc. Therefore, renewable sources utilization technology and bioenergy pro‐ duction technology developed fast for solving such two problems. Bioethanol as one of the biofuel has been applied in automobiles with gasoline in different blending proportions (Zhou and Thomson, 2009; Yan and Lin, 2009). Biobutanol is one of the new types of biofuel. It continuously attracted the attention of researchers and industrialists because of its several distinct advantages.