The Rhizoctonia blight causing by Rhizoctonia spp. is an important disease of turfgrass, requiring fungicide application to maintain acceptable conditions for turfgrass good qualities in the golf course. The experiment was conducted to determine the mean 50% effective concentration inhibiting mycelial growth (EC 50 ) value of to flutolanil, pyraclostrobine and hexaconazole to Rhizoctonia solani AG-1 IB, Rhizoctonia cerealis and Rhizoctonia solani AG2-2IV isolated from Gyeongbuk province of Korea in vitro. Five discriminatory concentrations of each fungicide were used to detect in vitro sensitivity. The mean of EC 5 0 values to three systemic fungicides was the lowest isolate of R. solani AG-1 IB. However, the sensitivity of fungicides to Rhizoctonia solani AG2-2IV were higher mean EC 50 value of 0.026 ㎍ a.i. ml-1of pyraclostrobine and 0.044 ㎍ a.i. ml -1 of flutolanil. R. cerealis was the lowest sensitivity to hexaconazole which was an average EC 50 value of 0.022 ㎍ a.i. ml -1 . Inhibition of mycelial growth rate (%) by three combine fungicides using the EC 50 value of each fungicide was the highest R. solani AG2-2IV. Results of this study were may confirmed in vitro response fungicide of three Rhizoctonia species for control of Rhizoctonia blight in the field.