<p><strong>Background.</strong> The use of organic fertilization on grasslands is an option on the biological, economic and environmental sense.<strong> Objective</strong>. In order to evaluate the poultry manure applied as a fertilizer in Kikuyo pastures (<em>Pennisetum clandestinum</em>, Ex Chiov) with grazing cows.<strong> Methodology. </strong>An experiment was carried out on a 21.6 ha farm in Ecuador, at 2° 13' and 78° 24' South and West, 2651 masl, rainfall of 1103 mm / year). Four fertilization levels corresponding to 0, 50, 100, 150 kg / ha / year, respectively, and load of 1.22 AU / ha were used. Irrigation was applied in the dry season. 21-25 Holstein cows with 526 kg of LW were used. Lactation was of 272 days. The time of occupation of a day and time of rest of 21-28 days. The concentrate offered was 0.4 kg / cow. The persistence of the pastures (%) and their yield per rotation in t ms / ha were determined. Heights and density and samples for dry matter, crude protein and crude fiber (%) were estimated. Milk/cow production data were recorded. ANOVA was used. Costs of the kg of milk produced were determined. <strong>Results</strong>. The significant differences (p &lt;0.05) were in F150, where the pasture increased up to 83% and the milk produced surpassed (p &lt;0.05) the other treatments. <strong>Implications</strong>. The treatment with the best response for grass and milk production was considered a positive reference to be recommended for producers in the zone. C<strong>onclusions</strong>. The contribution of 150 kg of manure + 50 kg / N2 / ha as urea, influenced in a higher nutrient intake, increased pasture persistence, yield and nutritional quality, with direct effects on the dairy response and reduced costs / kg of milk.</p>